外源性核酸对肝纤维化大鼠血浆中去甲肾上腺素和多巴胺的影响

Effect of Exogenous Nucleic Acid on Norepinephrine and Dopamine in Plasma of Rats with Hepatic Fibrosis

目的探讨外源性核酸对四氯化碳(CCl4)诱导的大鼠肝纤维化模型血浆中去甲肾上腺素(NE)和多巴胺(DA)的影响及其抗肝纤维化的可能机制。方法将30只Wistar大鼠随机分为正常组、肝纤维化模型组、外源性核酸处理组。苏木精-伊红染色,光镜下观察肝组织病理学改变,Van Gieson(VG)染色分析肝纤维化程度,同时用高压液相色谱-电化学(HPLC-ECD)法检测大鼠血浆中NE和DA水平。结果核酸组大鼠肝组织病理学改变较模型组有明显改善。模型组大鼠血浆DA含量[(0.39±0.19)ng/ml]显著低于正常组[(0.92±0.53)ng/ml](P<0.05),核酸组大鼠血浆DA的含量[(0.87±0.55)ng/ml]高于模型组(P<0.05)。大鼠血浆NE含量模型组[(1.13±0.77)ng/ml]和核酸组[(1.38±0.71)ng/ml]低于正常组[(4.38±2.77)ng/ml](P<0.05),核酸组与模型组NE的含量无明显差异(P>0.05)。结论外源性核酸对慢性肝损伤有一定的保护作用,可显著改善CCl4诱导的大鼠肝纤维化损伤。其可能的作用机制之一是通过调节肝纤维化大鼠血浆中NE的前体物质DA的含量,增加了交感神经的活性而起到抗纤维化的作用。

Objective To investigate the effect of exogenous nucleic acid on norepinephrine （NE） and dopamine （DA） in plasma of rats with hepatic fibrosis induced by carbon tetrachloride （CCl4） and the possible mechanism resisting hepatic fibrosis. Methods The 30 rats were randomly divided into control group, model group and nucleic acid-treated group. The NE and DA concentrations in plasma were determined by high performance liquid chromatography-electrochemical detector （HPLC- ECD）. Results The histopathological changes of liver in nucleic acid-treated group were significantly alleviated as compared with those in model group. The levels of DA in model group [（0.39±0.19） ng/ml] were significantly lower than in control group [（0.92±0.53） ng/ml] （P〈0.05）, while those in nucleic acid-treated group [（0. 87±0. 55） ng/ml] higher than in model group （P〈0.05）. The levels of NE in model group [（1.13±0.77） ng/ml] and nucleic acid-treated group [（1.38±0.71）ng/ml] were lower than in control group [（4.38±2.77） ng/ml] （P〈0.05）, but there was no significant difference between model and nucleic acid-treated groups （P〉0.05）. Conclusion Exogenous nucleic acid has protective effects on hepatic injury and can significantly alleviate the CCl4-induced hepatic fibrosis injury in rats. One of the possible mechanisms is to regulate the levels of plasma DA and NE in rats with hepatic fibrosis.