巢式甲基化特异性PCR检测p16基因甲基化

Detection of methylation of p16 gene by nested methylation specific polymerase chain reaction

目的利用巢式甲基化特异性PCR(Nested-MSP,nMSP)法检测非小细胞肺癌患者血清中p16基因的甲基化状态,探讨其在肿瘤早期诊断中的意义。方法利用nMSP法检测了65例非小细胞肺癌患者(其中鳞癌48例,腺癌17例)血清中p16基因的甲基化状态。结果在65例非小细胞肺癌患者血清中,p16基因的甲基化百分率为72.3%(47/65);nMSP法比普通的MSP法具有更高的灵敏度。结论巢式甲基化特异性PCR是一种灵敏度高、特异性强的甲基化检测方法,可广泛应用于基因甲基化分析。

Objective To investigate the aberrant hypermethylation of the p16 gene in plasma DNA of non-small cell lung cancer patients. Methods Tumor-associated aberrant hypermethylation of the p16 gene in plasma DNA from 65 non-small cell lung cancer （NSCLC） patients （48 squamous cell carcinoma （SCC） and 17 adenocarcinoma （ADC））were detected,using a modified nested methylation-specific PCR （nMSP）. Results p16 hypermethylation was present in 72. 3% （47/65） of the plasma DNA samples in tumor samples. This technique increased the sensibility of detecting p16 hypermethylation from DNA samples. Conclusion Study indicated that aberrant methylation of p16 may be a biomarker for the diagnosis of NSCLC patients, and nMSP is a reliable method for the purpose.