欧洲银叶椴种子快速解除休眠的技术

Techniques of Quickly Breaking Seed Dormancy of Tilia tomentosa

Silver Linden（Tilia tomentosa）seed has the character of complex dormancy.Based on the dormant mechanism of silver linden seed and the previous results with a single factor experiment on breaking seed dormancy,an orthogonal design L 27（3 13）with four factors（seeds shucked off pericarp dipping in water,GA3 treatment,6-BA treatment and stratification）and three levels was conducted to explore the optimal technical combination for quickly breaking seed dormancy,promoting germination and seedling vigorous growth.The result indicated:the optimal combination of factor and level was 1)shucking off pericarp by machine;2)scalding seeds for 2 s with 100 ℃ boiling water,then soaking in room temperature water for 24 h;3)soaking in 200 mg·L-1 GA3 and 6-BA solution,respectively for 24 h;4)stratification in varying temperature,i.e.warm temperature（10～15 ℃）for 5 d,then in low temperature（0～5 ℃）for 15 d.Seeds treated with this combination germinated after 20 d.The germinating rate in nursery garden was 71.2%,and the seedlings grew vigorously.