稀土元素对鲤鱼肝胰脏多种酶及MDA含量的影响

Effect of Rare Earth-element on the Enzymes and MDA in Liver and Pancreas of Cyprinus carpio Linnaeus

目的:探究稀土元素对鱼类肝胰脏抗氧化性及淀粉酶的长期影响。方法:将鲤鱼暴露于0.01、0.1、2、5mg/L的La3+溶液中120d,研究分析了La3+对鲤鱼肝胰脏POD和SOD活性,MDA含量及SOD和AMY同工酶的影响。结果:表明0.01、0.1、2、5mg/LLa3+组与对照组相比,镧对肝胰脏中MDA含量影响不显著,对SOD和POD的活性却表现出了显著抑制(p<0.05或p<0.01)。同工酶电泳实验结果表明:鲤鱼肝胰脏SOD酶带,对照组有3条,但处理组各有1条,且酶带亮度弱于对照;0.01、0.1mg/L组La3+导致AMY酶带亮度较对照组明显增强,且新增1条酶带。但2、5mg/L组,镧对AMY同工酶影响并不显著。结论:La3+能使肝胰脏SOD、POD活性减弱,能使SOD、AMY同工酶酶谱发生显著变化。

Objective：A long-term impact of rare-earth element on the antioxidant capability and amylase in liver and pancreas of fish was studied.Method：Cyprinus carpio Linnaeus（carpio） was exposed in 0.01 mg/L,0.1 mg/L,2mg/L,5mg/L La^3＋ solution for 120d to study effect of La^3＋ on activities of POD and SOD,contents of MDA,isozymes of SOD and AMY in liver and pancreas of carpio.Result：There was no significantly difference of MDA contents between treatment groups and control group,however,activities of SOD and POD were significantly inhabited by La3＋（p〈0.05或p〈0.01）.The results of PAGE indicated that there were 3 SOD bands in the control group,but 1 band respectively and the color was whiter in the treatment groups.Analysis of AMY isozyme showed that the bands of AMY isozyme in 0.01、0.1 mg/L treatment groups were not only darker in color but also produced one new band than in control group.However,La^3＋ expressed no effects on isozymes of AMY in 2,5mg/L treatment groups as compared to control group.Conclusion：Activities of POD and SOD were decreased and patterns of SOD and AMY isozymes influenced by La^3＋ in liver and pancreas of carpio.