利用PCR技术快速制备DNA分子量标准物

A Method for Fast Preparation of DNA Marker by PCR Technology

目的:制备一套DNA分子量标准物。方法:利用多聚酶链式反应技术(PCR),以连接有9个不同大小DNA片段的pMD18-T载体为模板,M13引物为通用引物,进行大规模扩增,并进行2%琼脂糖凝胶电泳分析和利用TanonGIS凝胶图像处理系统对凝胶扫描及拍照。结果:DNA分子量条带大小依次为1653bp、1173bp、691bp、606bp、496bp、401bp、326bp、238bp、155bp,条带分布适度、亮度清晰。结论:制备了能满足研究和实验室的要求DNA分子量标准物,和市售的相比,成本大大降低。

Objective：To make a serise DNA Marker.Method：With the template of pMD 18-T Vectors ligated 9 different sizes DNA fragment and the M13 currency primer,DNA Marker that adapted to laboratory was made by a large-scale PCR amplification.DNA Marker was electrophoresised in 2% agarose gels and was analyzed by Tanon GIS gel image disposal system.Result：the band size of DNA Marker is 1 653bp,1 173bp,691bp,606bp,496bp,401bp,326bp,238bp,155bp.The bands are even and clear.Conclusion：The price of made DNA Marker is much lower than the sales.It meets the need of research and laboratories.