三种淡水鱼消化道黏液细胞的组织化学染色

Histochemistry Staining of Mucous Cells on Digestive Tract of Three Species Freshwater Fish

目的:探索淡水鱼消化道黏液细胞的组织化学染色。方法:利用阿利新蓝和过碘酸雪夫试剂染色对鲶鱼(Silurus aso-tus)、鲢鱼(Hypophthalmichthys molirtix)、鲫鱼(Carassius auratus)消化道黏液细胞的类型与分布进行了观察,结果:AB-PAS(Alcian blue and periodic acid schiff’s reation)染色后动物消化管黏液细胞表现为4个类型:Ⅰ型玫瑰红色、Ⅱ型蓝绿色、Ⅲ型紫红色、Ⅳ型蓝紫色。除鲫鱼外动物食管始部黏液细胞多为圆形或椭圆形,食管中部黏液细胞主要是Ⅱ型和Ⅳ型杯状细胞。除鲫鱼动物胃体柱状黏膜上皮细胞与胃腺颈部细胞主要为Ⅰ型和Ⅲ型,黏液颗粒主要集中在细胞的核上区。链鱼、鲶鱼胃腺黏液细胞为Ⅰ型和Ⅲ型。链鱼、鲶鱼小肠的杯状细胞主要为Ⅲ型和Ⅳ型。鲫鱼小肠杯状细胞主要有Ⅱ型和Ⅳ型。这三种动物大肠杯状细胞主要为Ⅱ型和Ⅳ型。

Objective：To optimize the histochemical staining of mucous cells in the digestive tract of freshwater fish.Method：The type and the distribution of mucous cells in digestive tract of Silurus asotus,Hypophthalmichthys molirtix,Carassius auratus were described using the AB-PAS（alcian blue and periodic acid schiff＇s reation） combined staining.Result：The mucous cells have four types：typeⅠ,in rose-red colour,type Ⅱ,in green-colour,type Ⅲ,in purplish-red colour,type Ⅳ,in purplish-blue colour.In forepart of oesophagus of the animals except C.auratus,the mucous cells appear in the shape of sphericity or oval;and in middle part of oesophagus,the mucous cells are goblet cells,and have two types of Ⅱand Ⅳ.In the ventriculus of the animals except C.auratus,the mucosal epithelium and mucous neck cells of fundic gland are mainly type Ⅲ and type Ⅰ cells,the mucous granules are being concentrated in their shallow part;and mucous cells of fundic gland,cardiac gland,pyloric gland of H.molirtix,S.asotus are mainly type Ⅲ and typeⅠcells.In the small intestinum of H.molirtix,S.asotus,goblet cells are mainly type Ⅲ and type Ⅳcells,but the goblet cells of C.auratus are mainly type Ⅱ and type Ⅳcells.In the large intestinum of the three species animals,mucous cells are mainly type Ⅱ and type Ⅳcells.