顺铂、三氧化二砷对人卵巢癌细胞的作用研究

STUDY OF ARSENIC TRIOXIDE AND CISPLATIN ON HUMAN OVARIAN CANCER CELL

目的:探讨顺铂(DDP)、三氧化二砷(As2O3)对人卵巢癌细胞HO-8910的联合作用及其分子生物学机制。方法:采用形态学方法研究DDP、As2O3对细胞增殖的影响,免疫化学染色法检测基因表达。结果:1.联合作用时,As2O3浓度在1.5μmol/L以上、DDP浓度在2μmol/L以上时,有显著性差异;当三氧化二砷、顺铂浓度分别是12μmol/L、4μmol/L时,72h时癌细胞接近100%死亡。2.DDP、As2O3引起HO-8910细胞p53基因表达增强。结论:DDP、As2O3对人卵巢癌细胞HO-8910的生长有协同抑制作用;DDP、As2O3联合作用引起卵巢癌细胞凋亡的机制与p53基因表达增强有关。

Objective：The study was carried out to investigate the effects of cisplatin and arsenic trioxide on ovarian cancer cell line HO -8910 and relative mechanism. Methods：Morphological changes of cells were observed and photoed by inverted microscope. Immunocytochemical staining was used to examine the expression of gene. Results：1. The inhibitive effect of arsenic trioxide （ 〉 1.5μmol/L） combined with cisplatin（ 〉 2μmol/L） was obvious and the inhibitive degree was nearly 100% when the concentrations of arsenic trioxide and cisplatin were 12μmoL/L and 4μmoL/L. 2. Higher concentrations of arsenic trioxide and cisplatin premoted up - regulated p53 gene expressions. Conclusion：The inhibitive effect of arsenic trioxide combined with cisplatin was more obvious than either one used separately; the apoptosis mechanism of ovarian cancer cell line HO-8910 is related with upregulating of p53 gene expression.