p16、p53和c-erbB-2蛋白在膀胱移行细胞癌中的表达及意义

P16,P53 AND C-ERBB-2 GENE ESPRESSION IN BLADDER CAR CINOMA

目的:探讨p16、c-erbB-2和p53蛋白表达与膀胱移行细胞癌(TCC)病理分级、临床分期和转移的关系。方法:应用免疫组化SP法对75例膀胱癌组织中p16、p53及c-erbB-2蛋白表达进行检测。结果:75例膀胱癌中p16、p53及c-erbB-2的阳性率分别41.3%(31/75)、44.0%(33/75)和40.0%(30/75),p16和c-erbB-2蛋白在膀胱癌中的阳性率与肿瘤病理分级和临床分期有显著意义(P<0.05),p53和c-erbB-2阳性率与肿瘤临床分期及转移有密切的关系(P<0.01)。77.3%(58/75)肿瘤有上述蛋白的阳性表达,其中53.3%(40/75)的肿瘤同时有多个蛋白的阳性表达。结论:肿瘤的多因素分析比单因素分析更有价值,癌基c-erbB-2和抑癌基因p53、p16蛋白的表达异常及协同作用在膀胱癌的发生发展中起重要作用。

Objective： To investigate the expression of p16, p53 and c-erbB -2 proteins in transitional cell carcinoma（TCC） of urinary bladder and to analyze their correlation with tumor grade, clinic stage and metastasis. Methods：Immunohistochemical method was used to detect the pl6, p53 and c-erbB -2 gene expressions in 75 cases with the TCC of urinary bladder;Results：Positive rates of p16, p53 and c -erbB -2 in 75 cases of bladder cancer were 41.3% , 44.0% and 40.0% , respectively, p16 and c - erbB -2 gene expressions were related to thetumor grade and clinic stage （ P 〈 0.05 ） ; p53 and c - erbB - 2 gene expressions were related to the clinic stage and metastasis （P 〈 0.01 ）. Oncogenes and/or antioncogenes altered expressions were found in 77.3% cases of bladder carcinoma, and oncogenes and/or antioncogenes with multiple altered expressions were found in 53.3% cases. Conclusion： The analysis of multiple oncogene and/or antioncogene is more valuable than that of single oncogene and/or antioncogene. The altered expression of oncogene c - erbB - 2 and antioncogenes p16 and p53 might play a promotive role in the tumorigenesis and development of bladder tumors.