摘要
目的观察伊贝沙坦对单侧输尿管梗阻大鼠肾间质中PDGF-BB介导的肾间质纤维化的影响。方法制作大鼠肾间质纤维化模型,随机分为假手术组、模型组和伊贝沙坦治疗组,术后第7、14天分别处死各组中6只大鼠,检测大鼠肾功能、尿β2-微球蛋白,用免疫组化方法测定肾组织中α-平滑肌肌动蛋白(α-SMA)和血小板衍生生长因子(PDGF-BB)蛋白表达,动态观察肾脏病理变化。结果与输尿管梗阻核型(UUO)组比较,治疗组肾间质纤维化明显减轻(P<0.05),肾间质中α-SMA、PDGF-BB表达明显减少(P<0.05)。结论伊贝沙坦可通过下调肾间质PDGF-BB表达减轻肾组织间质纤维化。
Objective To investigate the effect of irbesartan on the expression of platelet-derived growth factor-bb（PDGF-BB） and its mechanism of renal interstitial fibrosis following unilateral ureteral obstruction（UUO） in rat kidney.Methods The rat models of renal interstitial fibrosis were established, and sham-operation, irbesartan treatment and control group were set up. 6 rats of each group were killed respectively at 7、14 days after surgery. Venous blood sample was collected to measure the levels of 24-hour ureal protein. The pathological changes and expression of PDGF-BB,α-smooth muscle actin（α-SMA） in renal interstitium were observed. Results Irbesartan could significantly decrease the expression of PDGF-BB（ P 〈0.05） and α-SMA（ P 〈0.05） in renal interstitium.Conclusion Irbesartan may play a protective role in the kidney by down-regulating PDGF-BB and alleviating renal interstitial fibrosis following UUO.
出处
《河北医药》
CAS
2008年第4期434-435,共2页
Hebei Medical Journal
基金
石家庄市科技支撑计划项目(编号:061461373)
关键词
肾间质
纤维化
伊贝沙坦
renal interstitium
fibrosis
irbesartan
