刺儿菜提取物抗BEL-7402肿瘤细胞活性的研究

INHBITION OF CEPHALANOPLOS(BUNGE) KITAM EXTRACT ON LIVER BEL-7402 CANCER CELLS

目的研究刺儿菜提取物对BEL-7402肿瘤细胞生长的抑制作用,观察提取物作用下肿瘤细胞的凋亡情况。方法利用MTT比色法测定提取物对BEL-7402肝癌细胞生长的抑制率;采用透射电镜观察法和TUNEL染色法研究了刺儿菜提取物诱导肝癌细胞凋亡。结果刺儿菜提取物10,20,40,80mg/L对肝癌细胞有明显的抑制作用,随提取物浓度的增加和培养时间的延长,抑制作用逐渐增强;对该提取液处理培养24,48,72,96h的细胞凋亡指数有显著差异。结论刺儿菜提取物对肿瘤细胞生长有抑制作用。

Objective To study the inhibition of Cephalanoplos（Bunge） kitam extract on human liver cancer cells. Method The inhibition of Cephalanoplos（Bunge） kitam extract on human liver cancer cells was measured with MTT colorimetic method, The human liver cancer cell apoptosis was studied by transmission electron microscope and TUNEL staining. Results The inhibition was enhanced with concentration increase of Cephalanoplos（Bunge） kitam extract and time of culture. With Cephalanoplos （Bunge） kitam extract 10, 20, 40, 80 mg/L for 24 ,48, 72 ,96h ,the cell apoptosis was significant by T test. Conclusion Cephalanoplos（Bunge） kitam extract can inhibit the growth of BEL-7402 cancer cells.