松仁油对高脂小鼠体重、血脂水平以及脂质过氧化的影响

INFLUENCE OF PINE-SEED OIL ON BODY WEIGHT,BLOOD FAT AND LIPID PEROXIDATION OF MICE FED BY HIGH FAT

目的探讨松仁油对喂食高脂饲料小鼠体重、血脂水平及脂质过氧化的影响。方法分别以高脂饲料(20%猪油和80%基础饲料,HF)及高脂饲料加松仁油(20%松仁油和80%高脂饲料,SHF)饲喂小鼠。于45d和90d测体脂含量及血脂水平,并取脑和肝脏制成匀浆液测丙二醛(MDA)、脂褐质(LP)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性。结果松仁油可影响小鼠体重并使高脂小鼠血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)含量降低,而高密度脂蛋白胆固醇(HDL-C)升高,提示松仁油具有调节血脂的作用。与正常组比,高脂小鼠脑和肝组织的CAT、总SOD和GSH-Px活性均显著降低,MDA和LP含量显著升高,提示高脂饲料可促进小鼠脑和肝组织脂质过氧化。松仁油组与高脂组比,小鼠脑和肝组织的CAT、总SOD和GSH-PX活性显著升高,MDA含量显著降低,提示松仁油可降低因高脂饲料引起的小鼠脂质过氧化。

Objective To study the influence of pine-seed oil upon body weight, blood lipid and lipid peroxidation of mice fed on high fat feed. Method The mice were fed by high fat feed （80% basal feed added 20% lard, HF） and HF added pine-seed oil （80% high fat feed added 20% pine-seed oil, SHF）. At 45 d and 90 d, the body fat content, blood lipid level, and malonyldehyde （MDA）, lipofusin （LP）, catalase （CAT）, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） of brain and liver homogenate were measured respectively. Results Pine-seed oil could reduce the body weight serum triglyceride （TG）, total cholesterol （TC）, low density lipoprotein-cholesterol （LDL-C） content, but increase high density lipoprotein-cholesterol （HDL-C） of the mice fed by HF. It suggested that pine-seed oil had definite effect on regulating blood lipid. Compared with normal control （NC） group, the activities of CAT, T-SOD, GSH-Px of brain and liver in HF group were remarkably lower, but MDA and LP remarkably higher. It suggested that HF could promote lipid peroxidation in brain and liver of mice. Compared with HF group, the activites of CAT,T-SOD and GSH-Px of brain and liver in SHF group were remarkably higher, but MDA remarkably lower. Conclusion Pine-seed oil can reduce the body weight, blood lipid, and lipid peroxidation of mice fed by HF.