染料木黄酮雌激素效应对辐射损伤小鼠生存率及免疫功能的影响

THE ESTROGENIC EFFECTS OF GENISTEIN ON SURVIVAL RATE AND IMMUNE RESPONSE IN IRRADIATED MICE

目的研究染料木黄酮(genistein,Gen)的雌激素效应对辐射损伤的防护作用。方法观察应用他莫西芬和Faslodex阻断受照小鼠雌激素受体活化情况下,Gen对接受全身致死剂量γ射线照射(7.5Gy)小鼠30d存活率、平均存活时间的影响,对接受4.0Gy全身照射小鼠非特异性和体液免疫的影响。结果Gen可以提高受照小鼠的存活率和平均存活时间,增强免疫功能,他莫西芬对小鼠生存率及免疫功能无明显影响,Faslodex降低受照小鼠的存活率和平均存活时间,抑制免疫功能。结论Gen通过激活ERβ途径,增强小鼠的抗辐射能力。

Objectives To study the radiation-protective effects produced by the estrogenic activities of genistein （Gen） . Method After blocking estrogen receptor activities by Tamoxifen and Faslodex, the effects of Gen on 30d survival rate and average life span of the mice were monitored after exposure to lethal dose 7 radiation （7.5Gy）, and the effects of Gen on innate （nonspecific） immune response as well as humoral （B-cell mediated） immune response of the mice were examined after exposure to 4.0Gy γ radiation. Results Gen can enhance the survival rate, average life span, and immune response of the γ-radiation exposed mice. While Tamoxifen had no significant effects on survival rate and immune response of the exposed mice, Faslodex caused a decrease in survival rate and average life span and also inhibited the immune response in those exposed mice. Conclusion Genistein can enhance the radiation tolerance in mice through activation of estrogen receptor 13 pathway.