大豆异黄酮对邻苯二甲酸二丁酯致小鼠遗传毒性的保护作用

THE PROTECTIVE EFFECTS OF SOYBEAN ISOFLAVONE ON GENETIC TOXICITY INDUCED BY DI-N-BUTUL PHTHALATE IN MICE

目的探讨大豆异黄酮(soybean isoflavone,SI)对邻苯二甲酸二丁酯(di-n-butyl phthalate,DBP)致小鼠遗传毒性的保护作用。方法(1)微核实验:7w龄雄性昆明种小鼠40只按体重随机分为4组:溶剂对照组、DBP模型组、低、高剂量SI实验组。将SI溶于0.5%羧甲基纤维素钠(CMC-Na)中配制成所需浓度灌胃SI实验组小鼠,溶剂对照组、DBP模型组每日灌胃等体积0.5%CMC-Na。连续给药或溶剂30d后,除溶剂对照组外,其他三组均灌胃给予DBP(0.5g/kgbw·d),连续处理5d,最后一次DBP处理后6h颈椎脱臼法处死小鼠,取胸骨胸髓涂片,计算微核细胞率。(2)精子畸形试验:6w龄雄性昆明种小鼠40只,分组情况及给药处理同微核实验。第一次DBP处理后35d颈椎脱臼法处死小鼠,取一侧附睾,计数精子数量、活动率、存活率及畸形率。结果低、高剂量SI实验组小鼠的微核率、畸形精子率与DBP模型组相比均显著降低,且精子活动率、存活率与之相比均有所升高。结论大豆异黄酮能够减轻DBP对小鼠导致的遗传毒性。

Objective To investigate the protective effects of soybean isoflavone （SI） on genetic toxicity induced by di-n-butul phthalate （DBP） in mice. Method （1） Micronucleus test： 40 male 7 w old Kunming mice were randomized into 4 groups： High and low dose SI intervention groups, DBP model group, and solvent control group. SI intervention groups were given different doses of SI （50,100mg/kg） for 30 d, meanwhile, the DBP group and solvent group were given 0.5 % sodium carboxymethyl cellulose. Then all groups were treated by 0.5g/kg DBP for 5d except solvent group. Mice were sacrificed 6 hour after last treatment, and then counting micronucleated cells in bone marrow. （2） Sperm malformation test： 40 male 6w old Kunming mice were grouped and treated the same as micronucleus test. Mice were sacrificed at 35 day after the first treatment, and then sperm quantity, motility, viability and abnormality rate were calculated. Result Micronucleus rate and sperm abnormality rate of SI intervention group were lower than DBP model group, while sperm motility and viability were higher than DBP model group. Conclusion SI can relieve the genetic toxicity induced by DBP in mice.