白癜风患者皮损NF—E2相关因子2的表达

Expression of NF-E 2 related factor in vitiligo lesions

目的分析白癜风患者皮损处Nrf 2表达水平。方法采用AEC免疫组化法检测4例表皮移植的寻常型白癜风患者皮损处Nrf 2的表达，蛋白印迹分析表皮移植的8例寻常型白癜风患者皮损组织和其正常供皮处组织细胞核和细胞质中Nrf 2的表达水平，并进行统计学差异分析。结果与白癜风患者正常供皮处组织相比，皮损处Nrf 2的表达多数集中在角质形成细胞的胞质中，细胞核中表达量非常低。蛋白印迹结果表明，白癜风患者皮损处核蛋白中Nrf 2表达水平（0．10±0．03）显著低于正常部位（0．26±0．03，P〈0．01），胞质蛋白中皮损处Nrf 2表达水平（0．61±0．03）与正常部位（0．60±0．02）差异无统计学意义（P〉0．05）。结论白癜风患者皮损处存在Nrf 2核转位异常。

Objective To investigate the level of NF-E2 related factor 2 （Nrf 2） in vitiligo lesions. Methods Tissue samples were obtained by press suction blisters at lesional and donor sites of 12 patients with vitiligo who were managed with epidermal transplantation. Four lesional samples from the patients were subjected to primary culture and the level of Nrf 2 was detected by AEC immunohistochemistry after 48 hours of culture. Western blotting was utilized to further detect the level of cytoplasmic and nuclear Nrf 2 in tissue samples from the other 8 patients with vitiligo. Results Immunohistochemistry revealed that Nrf 2 was predominantly expressed in cytoplasm, rather than nuclei, of keratinocytes in vitiligo lesions compared with the normal skin of patients. The level of nuclear Nrf 2 was significantly lower in lesions than that in normal skin （0.10 ± 0.03 vs 0.26 ±0.03, P 〈 0.01 ） of the patients. In contrast, there was no significant difference in the level of cytoplasmic Nrf 2 between lesional and normal skin （0.61 ± 0.03 vs 0.60 ± 0.02, P 〉 0.05） of patients. Conclusion These results reveal an abnormality of nuclear translocation of Nrf 2 in vitiligo lesions.