贵州省彝族、瑶族、汉族人群乙型肝炎病毒感染与白细胞介素-10—592多态性相关研究

Study on the association of IL-10-592 polymorphism with susceptibility to hepatitis B viral infection in Han, Yi and Yao ethnic groups in Guizhou province

目的研究贵州省世居少数民族（彝族、瑶族）及汉族人群白细胞介素（IL）-10基因多态性与HBV易感性的相关性。方法对研究人群采用PCR-RFLP方法分析IL-10基因启动子区．592位点多态性。结果贵州省黔西彝族、威宁彝族、荔波瑶族及汉族人群的IL-10-592位点基因型频率分别为：纯合突变型（AA）为51．43％、40．74％、32．53％、49．59％；杂合子（AC）41.9％、40．74％、54．82％、44．63％；野生型（CC）6．67％、18．52％、12．65％、579％。等位基因频率：A为59．94％～72．38％，C为27．62％～40．06％。IL-10-592基因型频率在威宁彝族、黔西彝族、荔波瑶族、荔波汉族间的差异有统计学意义（P〈0．05），其中荔波瑶族与黔西彝族、荔波瑶族与荔波汉族、黔西彝族与威宁彝族、威宁彝族与荔波汉族之间的差异有统计学意义（P〈0．05）。IL-10-592基因多态性在民族总体人群以及黔西彝族感染组与非感染组中的分布差异有统计学意义（P〈0．05）。结论IL-10基因启动子区-592位点多态性可能与贵州省汉、瑶、彝族人群的HBV易感性相关。IL-10基因多态性可能是HBV易感的危险因素之一。

Objective To investigate the association of IL 10 gene promoter polymorphism with susceptibility to hepatitis B viral infection in Han, Yi and Yao ethnic groups from Guizhou province. Methods Five hundred volunteers from Guizhou province were selected to undertake PCR-RFLP for detection of IL-10 gene promoter -592 polymorphisim. Results The genotypic distributions of IL-10-592 were 32.53%-51.43% （AA）,40.74%-54.82% （AC）, 5.79%-18.52% （CC） whereas the allelic frequencies were 59.94 % -72.38 % for the A allele, and 27.62 % -40.06 % for the C allele in Han, Yi and Yao ethnics from Guizhou. The distributions of allele and genotype frequencies of IL-10-592 were statistically different between Yao ethnic in Libo and Yi ethnic in Qianxi, Yao ethnic in Libo and Han ethnic in Libo, Yi ethnic in Qianxi and Yi ethnic in Weining, Yi ethnic in Weining and Han ethnic in Libo （P 〈 0.05）. IL-10-592 polymorphism was associated with HBV infection in Yi ethnic in Qianxi and the whole population. Conclusion IL-10-592 gene polymorphisms influenced the susceptibility to HBV infection in Han, Yao, Yi sub-populations in Guizhou. Result of the study suggested that IL-10-592 gene polymorphisms might serve as a risk factor to HBV infection.