马尾树愈伤组织的诱导与褐化控制研究

Experimental Study on the Induction and Browning Control of Rhoiptelea chiliantha Callus

[目的]为保护和开发利用马尾树资源提供科学的理论依据。[方法]以MS为基本培养基,用IBA和6-BA以及不同浓度配比的抗褐化剂AC、VC和Na2S2O3诱导马尾树愈伤组织。[结果]在诱导过程中马尾树愈伤组织的褐化现象很突出,接种几小时后外植体周围即出现褐化物质。以冬芽和春芽为外植体时,这些抗褐化剂均不起作用。以嫩叶为外植体时,抗褐化剂AC起显著作用。3种抗褐化剂组合使用时,仅AC的作用显著,而VC和Na2S2O3的作用不显著。仅在以嫩叶为外植体的培养中诱导出了愈伤组织。MS+IBA 1.5mg/L+6-BA2.5 mg/L+AC3.0 g/L最适于马尾树嫩叶愈伤组织的诱导。[结论]马尾树的嫩叶可用于诱导愈伤组织,这为马尾树的细胞培养奠定了基础。

[Objective] The purpose was to supply scientifically theoretical basis for protecting and exploiting Rhoiptelea chiliantha Dieis et Hang-Mazz resources. [Method] With MS as basic media, IBA, 6-BA and anti-browning agents AC, Vc and Na2S2O3 with different conch, ratios were used to induce R. chiliantha callus. [Result] The browning phenomenon ofR. chiliantha callus was very outstanding in the induction course and the browning substance appeared around the explant after several hours since inoculation. With winter and spring buds as explants, these anti -browning agents bad no effect. With tender leaves as explants, the anti -browning agent AC bad significant effect. When the combination of these 3 anti -browning agents was used, only the effect of AC was significant and that of Vc and Na2S2O3 were not significant. Only in the culture with tender leaves as explants, the callus was induced. MS ＋ IBA 1.5 mg/L ＋ 6-BA 9.5 mg/L ＋ AC 3.0 g/L was optimum for the callus induction of tender leaves in R. chiliantha. [Conclusion] The tender leaves of R. chiliantha could be used to induce the callus, which laid a foundation for the cell culture of R. chiliantha.