黄芩总黄酮·黄芩苷提取及纯化工艺研究

Study on Extraction and Purifiction Process of Total Flavonoid and Baicalin in Scutellaria baicalensis

[目的]确定黄芩总黄酮与黄芩苷提取及纯化的最佳工艺条件。[方法]以黄芩总黄酮与黄芩苷得率作为评价指标,采用单因素及正交试验进行优选。[结果]最优工艺为:取黄芩粗粉,加10倍量水,煎煮2次,1.0 h/次;离心所得上清液调pH值至1.5,60℃保温30 min,所得沉淀加适量水搅匀,调pH值至中性,加等量乙醇醇沉;上清液再调至pH值为1.5,60℃保温30 min,静置、离心所得沉淀,水洗至中性,60℃真空干燥得黄芩提取物。其中,黄芩总黄酮、黄芩苷含量分别可达94.15%、80.15%。[结论]该工艺合理、可行,适合黄芩总黄酮、黄芩苷的工业化生产。

[Objective] This paper aimed to determine the optimum process condition of extraction and purifiction of total flavonoid and baicalin in Scutellaria baicalensis. [Method] Taking the extraction rate of total flavonoid and baicalin in Scutellaria baicalensis as evaluation index, single factor and orthogonal test were used for optimal selection. [Result] The optimum process was as follows： 10 times volume of water was added into coarse powder of Scutellaria baicalensis. The mixture was decocted for 2 times with 1.0 h / times, and then centrifuged. The supernatants were adjusted to pH 1.5, and stored at 60 ℃ for 30 min. After blending the precipitation with water, the mixture was adjusted to neutral pH and 50% alcohol was used as alcohol-precipitation. The supernatants were adjusted to pH 1.5 again and stored at 60 ℃ for 30 min. The standing precipitation was centrifuged, and then washed to neutral pH. Scutellaria baicalensis extraction was obtained by vacuum drying at 60 ℃. The contents of total flavonoid and baicalin in Scutellaria baicalensis were 94.15% and 80.15%, respectively. [Conclusion] This process was rational, practical and adapted to industrialized production of total flavonoid and baicalin in Scutellaria baicalensis.