利用层析法纯化鹿胎盘多肽的研究

Research of Purifying the Deer Placenta Polypeptide by Chromatography

利用离子交换柱及凝胶层析柱对鹿胎盘多肽进行纯化,试验结果表明,鹿胎盘多肽粗提溶液利用Q- Sepharose Fast Flow柱,用5 mmol/L pH 8.0的Tris-HCl缓冲液洗脱得到3个组分,经E-玫瑰花环试验证实,第2个组分生物活性较高,使脱受体的人体T淋巴细胞的E-玫瑰花环率恢复到62.5%。将分离出的较高活性组分经Sephadex G-50凝胶层析柱,用50 mmol/L的NaCl缓冲溶液洗脱,得到2个组分,第2个组分具有较高活性,使脱受体的人体T淋巴细胞的E-玫瑰花环率恢复到72.5%,其特征吸收峰在228 nm处。

We utilized the ion exchange column and zeolites column to purify the deer placenta polypeptide in the test. Test result showed that we could separate the deer placenta polypeptide solution by Q-Sepharose Fast Flow column, and buffered it with Tris-HCl of 5mmol/L pHS. 0 liquid elute to get 3 components. It is verified that the biological activities of the second component is relatively high by the garland test of E- rosette. E-rosette rate of human T lymphocyte after off the receptor went back to 62.5%. Two highly active components can be separated out by isolated by Sephadex G-50 column, and buffered with NaCl of 50mmol/L solution elute. The second component separated has higher activity through test of E-rosette. E- rosette rate of human T lymphocyte after off the receptor went back to 72.5%. The T lymphocyte absorbing peak locates at 228nm.