甘草内生细菌多样性研究

Diversity of Endophtic Bacteria Isolated from Glycyrrhiza

以分离培养的方法对内蒙古鄂尔多斯市甘草基地野生及栽培甘草内生细菌的多样性进行了初步研究。结果表明,野生及栽培甘草植株内存在大量种群丰富的内生细菌。经ERIC-PCR指纹图谱分析,共分离到120株内生细菌,野生及栽培甘草均表现出根和叶部位的内生细菌数量多于茎部。对其中82株进行16SrDNA片段测序分析,结果表明这些内生细菌分别与GenBank中α、β、γ-Proteobacteria、Firmicutes、Actinobacteria五类细菌中的19个已知属相似性达到97%～100%。内生细菌的主要优势种群为芽孢杆菌属(Bacillussp.)、假单胞菌属(Pseudomonassp.)、泛菌属(Pantoeasp.)和沙雷氏菌属(Serratiasp.)。

120 strains of endophytic bacteria identified by ERIC-PCR were isolated from wild and cultivated Glycyrrhiza uralensis plants which collected from Erdos Innermongolia province. The identified results indicated that Glycyrrhiza uralensis plants has plenty of endophytic bacterium in density and population, and the density is higher in root and leave than in stem. Partial sequence analysis of 16S rDNA gene of 82 strains indicated that these strains were in a high similarity with 19 known genus which belong to α、 β、γ-Proteobacteria 、 Firmicutes and Actinobacteria. The dominant genus were Bacillus sp., Pseudomonas sp., Pantoea sp. and Serratia sp..