兔后肢缺血模型的制作

A hindlimb ischemia model in rabbits.

目的建立患肢缺血实验动物模型。方法结扎10只家兔左后肢的股动脉,1周后切除髂外动脉,股动脉及所有侧支血管。术后6周内定期评价每只家兔缺血程度。结果术后临床观察4只家兔患肢有缺血表现(3只皮肤发绀,1只足趾坏死);患肢血压术后3d下降明显(P<0.05,VS术前),以后缓慢上升,到术后6周达到平台期。放射性同位素灌注扫描显示了与血压类似结果。术后2周患肢造影,髂内动脉发出侧支血管与腘动脉发生重建。术后6周造影,侧支血管增粗扭曲。结论成功制作家兔后肢持续缺血模型。

Objective To establish an experimental animal model of limb ischemia. Methods In the left hindlimb of 10 New Zealand White rabbits, ischemia was induced by arteries excision from distal external iliac artery to superficial femoral artery and all collateral arteries after ligation of common femoral arteries 1 week later, and then both hindlimbs were serially examined to assess the ischemia according to the time table until postoperative 6 weeks. Results Clinical observation, blood pressure, radioisotopic perfusion scan and angiography were assessed. Clinical ischemic changes （cyanopathy, necrosis） of the operated limbs were observed in 40%. The blood pressure of the left calves was measurable on postoperative day 3 （P 〈 0.05, VS preoperative day） and then gradually increased to reach a plateau in postoperative week 6.Radioisotopic arterial perfusion showed similar profiles as in blood pressure. Angiography of ischemia hindlimbs demonstrated a few collateral vessels arising from the internal ilial artery with the reconstitution of popliteal artery in postoperative week 2.In postoperative week 6, collaterals remained the same in number. However, these vessels became dilated and tortuous, and showed reconstitution in distal hindlimb. Conclusion Persistent hindlimb ischemia model in the rabbit was made successfully.