STAT3 siRNA引起U251细胞凋亡的研究

Knockdown of STAT3 expression by siRNA induces apoptosis in U251 cell line

目的:观察利用siRNA技术下调人脑胶质母细胞瘤细胞(U251)STAT3表达后对细胞凋亡的影响。方法:分离培养并间接免疫荧光鉴定大鼠原代星形胶质细胞(NA),用STAT3干涉质粒载体(pSilence2.1-STAT3)及对照载体(pSilence2.1-GFP)转染U251细胞及NA细胞,用Hoechst 33258染色观察凋亡的形态学改变,用Annexin-V试剂盒定量检测凋亡。结果:分离培养了符合实验要求的NA细胞,转染pSilence2.1-STAT3后,U251细胞出现了明显的时间依赖性细胞凋亡,而NA细胞无显著凋亡发生。结论:pSilence2.1-STAT3能诱导U251细胞凋亡,而对正常细胞无显著影响。

Objective：To observe the effect of knockdown of STAT3 expression by RNA interference on apoptosiof U251 cell line. Methods：Primary astrocytes （NA cells） were isolated and identified by indirect immunofiuorescence. The changes of apeptotic morphology were observed by Hoechst 33258 stain after U251 cells or NA ceils were treated with STAT3 interference vector （pSilence2.1-STAT3）or the control interference vector（ pSilence2.1-GFP）, and the level of apoptusis was quantitated by Annexin-V kit. Results：NA cell lines were obtained. A significant time-dependent apoptosis was observed in U251 cell line treated with pSilence2.1-STAT3 vector. However, PSilence2.1-STAT3 vector did not affect significantly the apeptosis level of NA cell line. Condusion：Apeptosis is induced significantly by pSilence2.1-STAT3 vector only in U251 cell line, but not NA cell line.