体外诱导的同种反应性淋巴细胞输注对大鼠皮肤移植存活时间的影响

Infusion of Alloreactive Lymphocytes Induced in vitro Can Prolong The Survival of Skin Allograft in Rats

目的观察体外诱导的同种反应性淋巴细胞输注是否可以诱导大鼠皮肤移植免疫耐受,从而探索一条诱导器官移植耐受的新途径。方法以供者Lewis大鼠淋巴细胞作为刺激物,体外刺激受者BN大鼠淋巴细胞增殖,应用CFSE法检测增殖情况;以增殖后的BN大鼠淋巴细胞作为自身独特型疫苗,输注给同品系的BN大鼠,并行Lewis到BN的皮肤移植,观察皮肤移植物生存期。结果同种反应性淋巴细胞体外能够活跃增殖;同种反应性淋巴细胞输注可以显著延长皮肤移植物的存活时间(P<0.05)。结论体外诱导的同种反应性淋巴细胞输注有可能成为诱导抗原特异性免疫耐受的一种新途径。

Objective To investigate whether infusion of alloreactive lymphocytes induced in vitro can prolong the survival of skin allograft in rats, so as to, find out a new method to induce transplantation tolerance. Method Lymphocytes from Lewis rat were used as stimulating cells, lymphocytes from BN rat were stimulated by these stimulating cells and expanded in vitro; the proliferation of BN lymphocytes were detected by CFSE method. The proliferated BN lymphocytes were used as idiotype vaccine to vaccinate BN rats, then skin allograft from Lewis rats were transplanted to BN rats. Results Alloreactive lymphocytes proliferated vigorously in vitro; infusion of alloreactive lymphocytes induced in vitro can prolong the survival of skin allograft significantly in rats. Conclusion Infusion of alloreactive lymphocytes induced invitro may be a new pathway to induce donor specific transplantation tolerance.