溶胶凝胶自组装膜改性纯钛表面接枝活性粘附肽

Immobilizing Bioadhesive Peptide onto the Ti by Sol-Gel Coating Technique and Self-Assembled Monolayer

为了改善植入材料与组织界面的生物反应性,本实验采用溶胶-凝胶及有机硅烷自组装膜技术对纯钛表面改性,固定促成骨细胞粘附生长的活性肽序列GYRGDS,观察改性后的材料表面微形貌、元素组成、润湿性等理化特性。结果表明粘附肽成功接枝到亲水性材料表面并在细胞培养早期发挥显著的促成骨细胞粘附功能,改善了钛与组织细胞的生物反应性。

To improve the biocompatibility of implant biomaterials, a short bioadhesive peptide GYRGDS aimed at cell integrin receptors is covalently binded to the titanium surface modified by self-assembled monolayers and sol-gel coating technique. The surface properties of the modified Ti including wettability, chemical composition and morphology were investigated systematically using water contact angle measurement, X-ray photoelectron spectroscopy （XPS）, scanning electron microscopy （SEM） and the fluorescein microscope. The efficiency of this bioreactive surface in promoting cell colonization was demonstrated by measuring the adhesion of osteoblast.