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非甾体类抗炎药NS398对前列腺癌细胞株DU145中RECK基因表达的调控 被引量：3

Nonsteroidal Anti-inflammatory Drug NS398 Regulates the RECK Gene Expression in the Prostate Carcinoma Strain DU145
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摘要 目的:通过检测非甾体类抗炎药NS398对前列腺癌细胞株DU145中RECK基因表达的调控,探讨其可能的抗肿瘤机制。方法:实验组(3组)分别以不同浓度(10、100、1000μmol/L)NS398作用于前列腺癌细胞株DU145,而对照组则无NS398,各组均培养48h后收集细胞,抽取各组细胞总RNA,检测RECK基因与MMP9的表达;抽取各组细胞总蛋白,应用Western印迹方法检测RECK蛋白的表达。结果:实验组细胞株中RECK基因表达均较对照组明显升高,其中以100μmol/L组升高最明显,而MMP9的含量明显降低;实验组RECK蛋白的表达也较对照组明显升高(P<0.05)。结论:NS398有明显的诱导RECK基因表达的作用,是其可能的抗肿瘤作用机制。 Objectives： To investigate the regulative effect of the nonsteroidal anti-inflammatory drug NS398 on the RECK gene in the prostate carcinoma strain DU145. Methods ： DU145 was treated with various concentrations of NS398 for 48 hours. The mRNA level was measured by RT PCR technique and the expression of the RECK protein determined by Western blot. Results ： The mRNA level of the RECK gene was obviously higher, while the MMP9 level markedly lower in the treated group than in the control, and so was the expression of the RECK protein. Conclusion ： NS398 induces the expression of the RECK gene, which might be the mechanism of its anti-tumor effect.
作者 徐振宇 高建平 孙颖浩 张征宇 葛京平 许传亮 王林辉
机构地区 南京军区南京总医院泌尿外科 上海第二军医大学长海医院泌尿外科
出处 《中华男科学杂志》 CAS CSCD 2008年第4期303-306,共4页 National Journal of Andrology
基金 国家杰出青年资金资助(30225046)
关键词 NS398 非甾体类抗炎药 前列腺癌 RECK基因 基质金属蛋白9 NS398 nonsteroidal anti-inflammatory drug prostate carcinoma RECK gene MMP9
分类号 R737.25 [医药卫生—肿瘤]
  • 相关文献

参考文献14

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二级参考文献30

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共引文献15

同被引文献50

  • 1徐振宇,许传亮,孙颖浩.RECK基因在前列腺组织中的表达及其与基质金属蛋白酶-9的关系[J].中华实验外科杂志,2005,22(9):1146-1146. 被引量：5
  • 2徐振宇,高建平,张征宇,葛京平,许传亮,孙颖浩.基质金属蛋白酶抑制剂RECK基因在前列腺细胞株中的表达及意义[J].中华男科学杂志,2005,11(10):727-730. 被引量：9
  • 3徐振宇,孙颖浩,高建平,张征宇,葛京平,许传亮,王林辉.RECK基因在前列腺细胞株中的表达及其与MMP-2的关系[J].江苏医药,2007,33(4):372-374. 被引量：2
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引证文献3

二级引证文献10

中华男科学杂志
2008年 第4期

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