中华植生藻某些特性的研究

STUDY ON SOME CHARACTERISTICS OF RICHELIA SINICA

中华植生藻(Richelia sinica HB 58)是最近分离培养的一个固氮蓝藻新种。该藻能在水生111(HB111)无氮培养基中,在130μE/(m^2·s)下于4—8h内呈指数生长。在指数生长期间,当温度分别采用29℃,35℃和38℃培养时,生长速率均随温度增高而加快,以38℃时为最快;增代时间随温度增高而缩短,在38℃时10.5h为一代。用80%丙酮提取色素,其叶绿素a含量为5.14—6.55 mg/g干重。与固氮鱼腥藻(Anabaena azotica HB686)和稻田鱼腥藻(A. oryzae HB 13)相比,它们的色素吸收峰有一定差别;用0.00lmo1/L磷酸缓冲液提取色素,与固氮鱼腥藻吸收峰相似。藻胆蛋白含量因培养条件或藻生长状况差别变动较大,藻蓝蛋白含量在32.00—134.00 mg/g干重之间;别藻蓝蛋白含量在15.00—52.00mg/g干重之间。不同条件下多次培养,均未发现C-藻红蛋白吸收峰,可能不具C-藻红蛋白或含量甚微。该藻蛋白质含量为59.44%(干重),与螺旋藻(Spirulina)和鱼粉相近,高于鱼腥藻(Anabaena)、栅藻(Scenedesmus)、小球藻(Chlorella)和盐藻(Dunaliella)。有可能代替鱼粉作为家畜、家禽的饲料和水产品饵料的精料或添加成分使用,是一种有应用前景的藻类。

Richelia sinica HB 58 is a new species of nitrogen-fixing blue-green algae which were isolated and cultured recently. It grew in HB 111 nitrogen-free medium and under light in-tensitly 130 μE/(m2·s). Exponential growth phase was between 4-8h in our batch culture. The growth rate increased along with temperature raising. The generation time shortened as. culture grew at 29℃, 35℃ and 38℃ respectively. At 38℃, generation time was 10.5 h. When the pigments were extracted with 80% acetone, their absorption spectra were different from that of Anabaena azotica HB 686 and A. oryzae HB 13. However, when the pigments were extracted with 0.001 mol/L phosphat buffer, their absorption spectra were similar to that of A. azotica HB 686. Chlorophyll a concentration was about 5.14-6.55 mg/g dry weight. In various conditions, phycocyanin (PC) and alloghycocyanin (APC) concentrations varied between 32.00-134.00 mg/g dry weight and 15.00-52.00 mg/g dry weight respectively. No. C-phy-coerythrin (PE) absorption spectrum was found in this organism. Maybe there was no PE or only with trace amount. Protein content was 59.44% (dry weight) which is similar to that of Spirulina and fish meal, but higher than that of Anabaena, Scenedesmus, Chlorella and Du-naliella. So that biomass of Richelia sinica HB 58 will possibly be used as a substitute for fish meal in feed of livestock, poultry and aquatic products. It's an alga with useful prospects.