HPLC法测定补阳还五合剂中黄芪甲苷的含量

HPLC determination of astragaloside Ⅳ in Buyang Huanwu mixture

目的:建立补阳还五合剂中黄芪甲苷的含量测定方法。方法:采用 Supelco ODS C_(18)(4.6 mm×250 mm,5μm)色谱柱,以甲醇-水-四氢呋喃(90:6:4,含0.2%三乙胺)为流动相,柱温25℃,流速1.2 mL·min^(-1),检测波长230nm,测定黄芪甲苷苯甲酰化产物的量。结果:黄芪甲苷对照品溶液在1.1～70.4μg·mL^(-1)范围内的衍生化产物吸收值呈线性,相关系数为0.9999;平均回收率(n=6)为95.63%,RSD 为2.4%。结论:黄芪甲苷衍生物紫外吸收强,HPLC 灵敏度高,重复性好,测定结果准确,可用于补阳还五合剂中黄芪甲苷的含量测定。

Objective：To develop a method for the determination of astragaloside IV in Buyang Huanwu mixture. Methods： HPLC determination of derivatized astragaloside IV, column temperature was 25 ℃, in the HPLC analysis using the ODS reversed -phase column Supelco C is （4.6 mm × 250 mm ,5μm） was used, mobile phase was methanol-water-tetrahydrofuran （90： 6： 4,0.02% trietylamine contained） at the flow rate of 1.2 mL·min^-1,and the content was detected at the UV wavelength of 230 nm. Results： The linear range of derivatized astragaloside IV was 1.1-70.4μg·mLμ^-1（r=0.9999）. The average recovery （n=6） was 95.63 % with RSD of 2.4%. Conclusions： The method is accurate and delicacy, and the absorbance of derivatized astragaloside IV is high. It can be adopted for the determination of astragaloside IV in Buyang Huanwu mixture.