快速液相色谱在重组人胰岛素肽图分析中的应用

The application of fast HPLC analysis method in human insulin peptide mapping

目的:探讨快速 HPLC 分析方法在重组人胰岛素肽图测定中的应用。方法:采用 Shimadzu Pack XR—ODS(3.0 mm×75mm,2.2 μm)色谱柱;流动相 A 为0.2 mol·L^(-1)硫酸盐缓冲液(pH 2.3)-乙腈(90:10),流动相 B 为乙腈-水(50:50),进行梯度洗脱。检测波长为214 nm;柱温50℃;流速0.8 mL·min^(-1)。结果:人胰岛素酶解样品的各个片段能够很好地分离,分析时间显著缩短。结论:本法简便、快速,经济,灵敏度高。可应用于重组人胰岛素肽图的测定。

Objective：To discuss the application of fast HPLC analysis method in human insulin peptide mapping. Method：The column was Shimadzu Pack XR-ODS（3.0 mm ×75 mm,2.2 μm）,0.2 mol·L^-1 sulphate buffer - acetonitrile（90： 10） and acetonitrile-water（50： 50） were used as mobile phase A and mobile phase B ,respectively. Performing the gradient elution process. The detection wavelength was 214 nm, column temperature was 50℃ and flow rate was 0. 8 mL·min^-1. Results：The digested fragments of human insulin were well separated, analysis time was shorten remarkably. Conclusion：This method is simple, economic, rapid, high sensitive and reliable for testing of human insulin peptide mapping.