HPLC内标法测定化妆品中8种防腐剂

HPLC determination of eight preservatives in cosmetics

目的:选择桂皮酸(CA)作为内标,建立高效液相色谱法测定化妆品中山梨酸(SA)、苯甲酸(BA)、对羟基苯甲酸甲酯(PHBA—Me)、对羟基苯甲酸乙酯(PHBA—Et)、对羟基苯甲酸异丙酯(PHBA—iPr)、对羟基苯甲酸丙酯(PHBA—Pr)、对羟基苯甲酸异丁酯(PHBA—iBu)、对羟基苯甲酸丁酯(PHBA—Bu)8种防腐剂。方法:采用 Diamonsil ODS(250 mm×4.6 mm,5μm)色谱柱,以乙腈、水(磷酸调 pH=3.0)、四氢呋喃为流动相采用梯度洗脱,检测波长为254 nm,流速为1.0 mL·min^(-1),样品用无水乙醇-水(80:20,磷酸调 pH:2.5)提取。结果:在上述条件下8种防腐剂可以达到基线分离,各组分标准曲线的线性相关系数均为1.0,加样回收率为91.5%～106.4%,检出限为0.025～0.5μg。结论:该方法简便、准确、灵敏,适合化妆品中防腐剂的实际分析。

Objective：Selecting cinnamic acid （CA） as an internal standard, a high performance liquid chromatog-raphy was developed for the determination of eight preservatives in cosmetic, including sorbic acid （SA）, benzoic acid （ BA）, methyl-p-hydroxy benzoate （ PHBA - Me ）, ethyl-p-hydroxy benzoate （PHBA-Et） , isopropyl-p-hydroxy benzoate （PHBA-iPr）, propyl-p-hydroxy benzoate （PHBA-Pr）, isobutyl -p-hydroxy benzoate （PHBA-iBu）, butyl-p-hydroxy benzoate （ PHBA-Bu）. Method. The column was Diamonsil ODS （250 mm×4.6 mm,5 μm） ,with acetontrile,water（phosphate adjusted pH = 3.0） ,tetrahydrofuran as the mobile phase adapted gradient elution. The detection wavelength was 254 nm, The flow rate was 1.0 mL·min^-1. Samples were extracted by ethanol-water （80：20 volume ratio, phosphate adjusted pH=2.5）. Results：Under this condition the eight preservatives were baseline separated, the linear correlation coefficient were 1.0, the recoveries were 91.5%-106.4%, detection limits were 0. 025-0.5 μg. Conclusion ： This method is simple, accurate and sensitive. It can be applied to real cosmetics analysis.