鼠李糖乳杆菌YHOC137及其发酵乳的体外抗致突变作用

Antimutagenic effects of Lactobacillus rhamnosus YHOC137 and its fermented milk in vitro

乳酸菌及其代谢产物具有优良的生理功能。本文研究了一株产胞外多糖的鼠李糖乳杆菌L.rhamnosusYHOC137对诱变剂的吸附能力,利用艾姆斯实验模型考察了L.rhamnosusYHOC137发酵乳、发酵乳丙酮提取物和产物胞外多糖的体外抗致突变作用。诱变剂吸附实验表明,YHOC137活细胞对2-硝基芴(2-NF)和4-硝基喹啉-N-氧化物(4NQNO)的吸附率分别为38%和55%,YHOC137死细胞对2-NF和4NQNO的吸附分别为25%和50%。YHOC137发酵牛乳能够显著抑制由于2-NF和4NQNO导致的鼠伤寒沙门氏菌的回复突变(P<0.05),抑制率在38.5%～54.3%之间,L.rhamnosus YHOC137发酵牛乳丙酮提取物对回复突变的抑制率在8.0%～35.3%之间;L.rhamnosus YHOC137胞外多糖对2-NF和4NQNO导致的回复突变的抑制率分别为12.8%和46.0%。

Lactic acid bacteria and their metabo;ites are of excellent physiological functions. The absorption of mutagens by Lactobacillus rhamnosus YHOC137 cells was studied. In vitro antimutagenic effects of Lactobacillus rhamnosus YHOC137 and its cultured milk and exopolysaccharides were studied in the Ames test mode;. Experiments of mutagens absorption by lactic acid bacteria showed that the absorption ratios of 2-NF and 4NQNO by living L. rhamnosus YHOC137 cells were 38% and 55%, respectively. And those of 2-NF and 4NQNO by dead YHOC131 cells were 25% and 50%, respectively. L. rhamnosus YHOC137-fermented milk could significantly inhibit the reverse mutation of Salmonella typhimuriu induced by 2-NF and 4NQNO （P 〈 0.05）, and the inhibition ratio was between 38.5 %54.3 %. The inhibition ratio of 2- NF- and 4NQNO- induced reverse mutation by acetone extracts of YHOC137- fermented milk was between 8.0%-35.3%. While those by exopolysaccharides from L. rhamnosus YHOC137 were 12.8% and 46%, respectively.