冬虫夏草发酵条件的研究

Study on fermented condition of Cordyceps sinensis

通过对冬虫夏草菌Cs11的深层发酵条件如pH、接种量、装液量、转速及其培养基碳源、氮源和无机盐的研究,得出其最适培养条件为24℃、170r/min、150mL/500mL、pH6.5,接种量10%,菌丝生长的最佳培养基:葡萄糖4%,豆饼粉2%,KH2PO40.2%,MgSO4.7H2O0.075%。发酵结果得到虫草菌丝粉中有效成分含量:腺苷>0.16%;甘露醇>8.0%。

The fermented conditions of Cordyceps sinensis including original pH,the quantity of inoculation, poured solution volume, rocking bed rate of rotation and medium composition including carbon sources, nitrogen sources and inorganic salt were studied. The results showed that the optimum fermented conditions were 24℃, 170r/min, pH6.5,inoculun quantity 10%. The optimum conditions were as follows： glucose 4%, bean cake 2%, KH2PO4 0.2% ,MgSO4 · 7H2O 0.075%. The contents of the active components in Cordyceps sinensis were manna alcohol 〉8.0% and adenosine 〉0.16%.