广佛手多糖分离提取的工艺优化

Optimized technique of extraction of Bergamot polysaccharide

通过单因素和正交实验优化超声波法提取广佛手多糖的实验条件,结果表明:采用超声波,在功率400W、预处理广佛手浆25min后,于料液比为1:30、90℃热水中浸提4h,得到广佛手粗多糖,多糖得率和纯度分别为3.18%和65.8%;再经多次分离纯化,可得到纯度高达86.48%的广佛手多糖。

The extraction technique of Bergamot polysaccharide by ultrasonic wave were optimized with single factor and orthogonal experiments. Results showed that Bergamot crude polysaccharide could obtain by ultrasonic wave power at 400W for 25min, with adding 30 times water and soaking for 4h at 90℃, polysaccharide extraction and purity separatively 3.18% and 65.8%, then many times centrifugalization, settling and purification, the purity of Bergamot polysaccharide attained 86.48%.