生物合成γ—氨基丁酸工艺条件的优化

Research on optimization of technology conditions of γ-amino butyric acid by biosynthesis

为了建立L-谷氨酸与γ-氨基丁酸(GABA)之间的转化关系,采用二次回归正交旋转组合设计方法对影响GABA生物合成的因素进行优化。建立了GABA生成量与底物浓度、温度、pH之间的数学模型;并确立了GABA制备的最佳工艺条件:底物浓度56.82mmoL/L、温度为40℃、pH为3.53。在此条件下,GABA的生成量达到0.84mg/mL。

For establishing the transformed relations between L-glutamic and γ-amino butyric acid, orthogonally rotational combination design was used to investigate biosynthesis technology parameters of γ-amino butyric acid. The mathematics matrix was established between the productions of γ-amino butyric acid （GABA） and three factors （substrate concentration, temperature and pH value）, The optimum technology conditions were as follows： substrate concentration. 56.82mmoL/L, temperature： 40℃ and pH value：3.53 and production of GABA was 0,84mg/mL under this conditions,