摘要
采用超声技术提取紫苏不同部位中乌索酸和齐墩果酸,建立RP-HPLC法测定其含量。色谱柱为Kromasil C18柱(250mm×4.6mm,5μm),流动相为甲醇-水(87:13),光电二极管阵列检测器,检测波长210nm,流速0.8mL/min,柱温28℃。乌索酸进样量在0.359～5.744μg,齐墩果酸进样量在0.174～2.784μg时呈现良好的线形关系,平均加样回收率分别为98.53%和98.36%,RSD分别为1.3%和1.5%。方法灵敏、准确,重现性好,可用于紫苏中乌索酸与齐墩果酸含量的测定。
Ursolic acid and oleanolic acid from Perillalinn were extracted by supersonic technology, and RP-HPLC method for the determination of ursolic acid and oleanolic acid was developed. Ursolic acid and oleanolic acid was separated and analyzed on Kromasil C18 column （250mm ×4.6mm, 5μm） using methanol-water（87：13） as mobile phase at flow rate of 0.8mL/min. UV detection was at 210nm and the column temperature was 28℃. An average recovery of 98.53% was obtained with a RSD of 1.3% in the range of 0.359-5.744μg for ursolic acid, and an average recovery of 98.36% was obtained with a RSD of 1.5% in the range of 0.174-2.784μg for oleanolic acid. HPLC method was proved to be sensitive, fast and accurate It can be used for the determination of ursolic acid and oleanolic acid in Perillalinn.
出处
《食品工业科技》
CAS
CSCD
北大核心
2008年第4期284-286,共3页
Science and Technology of Food Industry
基金
国家“863”计划重点资助项目(2002AA2Z3217)
