PAI-1 4G／5G基因多态性与严重急性呼吸综合征后骨坏死

Relationship between Post-SARS osteonecrosis and PAI-1 4G/5G gene polymorphisms

[目的]测定严重急性呼吸窘迫综合征(SARS)后骨坏死患者低纤溶倾向的纤溶酶原激活抑制物PAI和相关基因PAI-4G/5G基因多态性与国人SARS后骨坏死的关系,以便于非创伤性骨坏死的早期诊断和易感人群的筛选。[方法]取61例SARS后骨坏死患者抽取空腹肘静脉血。另取健康人群52名为对照,应用凝血仪、酶联免疫吸附(ELISA)方法测定PAI值,应用PCR+固相寡核苷酸连接分析(SPOLA)法测定PAI-1基因4G/5G多态性。[结果]SARS后骨坏死患者PAI异常明显PAI(15·64±13·85)U/mlVS(7·96±4·27)U/ml,PAI-1基因4G/4G在骨坏死人群中增高,但统计学未见差异性,血浆PAI活性与4G/4G纯合子基因型密切相关。[结论]SARS后骨坏死患者的凝血指标异常,PAI可作为骨坏死易感因素的筛选指标。PAI-1基因4G/4G可能是国人骨坏死的遗传标记。

[Objective] To explore the correlation between and post -severe acute respiratory symptom （SARS） patients with osteonecrosis, investigate the etiology of post - SARS osteonecrosis, and select the sensitive molecular symbols for early diagnosis and distinguish the high risk population. [ Method] The studied subjects were divided into two groups. Totally 62 post - SARS patients with osteonecrosis was one group, and 52 age and sex matched healthy people was as normal controlled group. Empty stomach blood samples from cubital veins were collected from both groups. Plasminogen activator inhibitor （PAI） by means of evzyme linked immunosorbent assay （ELISA）, and PAI - 1 4G/5G polymorphism was detected by polymerase chain reaction PCR and Solid phase oligonucleotide assay （SPOLA） . [ Result ] The blood agents of post - SARS patients changed obviously with PAI - 1 polymorphism detected in post - SARS group was more than that of the control group, but had no statistical significance. The plasma PAI activity was related to homozygote 4G/4G genotype. This reveals that homozygote 4G/4G genotype maybe a susceptible gene mark to Chinese osteonecrosis patients. [ Conclusion] PAI - 1 is sensitive blood symbol for screening high - risk susceptible population. 4G/4G PAI - 1 genotype may be an etiological factor in osteonecrosis.