曼氏无针乌贼墨囊组织学及墨汁形成的超微结构

Histology of the ink sac of cuttlefish Sepiella maindroni and ultrastructure of the ink formation

采用组织学和电镜技术对曼氏无针乌贼的墨囊及墨腺细胞进行了研究。结果表明:墨囊壁和导管壁由外膜、肌肉层和黏膜三部分组成;墨腺体集中在墨囊底部,呈索状,腺体中部含丰富的结缔组织;墨汁颗粒以游离态形式分布于索状腺体的间隙及墨囊腔中。实验观察到无分泌黑色素功能的A型细胞和有分泌黑色素功能的B型细胞;在B型细胞中可见黑色素颗粒储存在囊泡中,囊泡在移出细胞的过程中逐渐变大,泡内的黑色素颗粒逐渐变多。囊泡可能通过胞吐的方式排出细胞外,黑色素排出后以颗粒的形式游离于细胞间隙中,形成墨汁。

The histology and ultrastructure of the ink sac of Sepiella maindroni de Rochebrune were studied using light and electron microscopy. Results showed that the ink sac of Sepiella maindroni opened into the end of the recta near the anus; the wall of the ink sac and its opening consisted of three parts： an endocardium, a muscle layer and a mucosal layer; the ink glands concentrated at the bottom of the ink sac were formed by the hyperplasia of the mucosal epithelium to the cavity of the ink sac, shaped as rope with abundant connective tissue in the centre. Type A cells which can not secrete melanin and type B cells which can secrete melanin were observed; melanin granules were seen only in the type B cells. The melanin was deposited in the vesicles. During the vesicles moving out of the cells, they became big and the melanin increased gradually. After that, the melanin granules were released in the intercellular space. The vesicles may be released with exocytosit. Then ink formed