芦荟对老年便秘小鼠肠运动功能的影响

Effect of aloe on intestine motility in the old costive mice

目的观察芦荟对老年便秘小鼠排便作用及肠运动功能的影响，并探讨其作用机制。方法高效液相色谱法测定芦荟粉中芦荟苷的含量，给老年小鼠灌服复方地芬诺酯混悬液，建立便秘模型，通过观察小鼠首次排黑便时间、粪便性状、粪便含水率及小肠墨汁推进百分率，评价芦荟对老年小鼠排便作用及肠运动功能的影响，并测定小鼠血清一氧化氮（NO）含量，探讨芦荟促进肠运动的机制。结果与模型对照组比较，芦荟低、中、高剂量组均能不同程度缩短便秘小鼠的首便时间[分别为（259．4±49．2）、（195．1±38．1）、（180．4±43．5）、（77．9±37．6）min]，增加6h黑便粒数及粪便含水率，芦荟中、高剂量组均能提高小鼠的小肠墨汁推进率。芦荟高剂量组血清NO含量与模型对照组比较[分别为（66．21±13．70）μmol／L与（84．67±22．94）μmol／L]，差异有统计学意义（P〈0．05）。相关性分析表明，血清NO含量与小肠墨汁推进率呈负相关（r=-0．346，P〈0．05）。结论芦荟能增加老年便秘小鼠的肠运动和粪便含水率，起到治疗便秘的作用，其作用机制可能与调节体内NO水平有关。

Objective To observe the effect of aloe on intestine motility in the old costive mice and investigate the mechanism for aloe promoting an intestinal motility. Methods The content of aloin in aloe powder was determined by high performance liquid chromatography （HPLC）. The old mice aged 15 months were randomly divided into 6 groups（10 in each group） ： blank control, positive control, constipation model, low-dose aloe, middle-dose aloe, high-dose aloe plus model. The mice of low, middle and high dosegroups were administered with aloe at a dosage of 0. 17 g/kg·body weight （bw）, 0.33 g/kg·bw, 1.00 g/kg·bw respectively for 7 days by gavage. The mice in positive control group were given 2.5 mg/kg·bw cisapride, and those in control and model group were treated with equivalent volume of distilled water. On the eighth day, the mice except control group were given Compound Diphenoxylate to establish constipation model. With the black Indian ink as marker, the first time of black stool discharge, the character and weight of the stool, and the ink propulsion rate by intestines in mice were observed respectively. The serum level of nitric oxide （NO） was determined by spectrophotometry. Results The content of aloin in aloe powder was 0. 266%. Compared with constipation model group, aloe groups in different dose decreased the first black stool time and increased stool grains and weight in 6 hours of constipated mice. The ink propulsion rates of intestines in the aloe groups were significantly higher than that of model group as well. The NO level in high-dose aloe group decreased more significantly compared with model group（P〈0.05）, and there was a negative correlation between the serum NO level and propulsion rate of intestines （ r = -0. 346, P〈0. 05） . Conclusions Aloe could promote the mobility of intestine and ameliorate the constipation of mice, which might attribute to the decrease of the serum NO level.