光动力法制备抗小鼠H22肝癌肿瘤疫苗的实验研究

Generation of Antitumor Vaccines for H22 Tumor on Mouse Using Photodynamic Therapy

目的:探讨PDT法制备的抗小鼠H22肝癌肿瘤疫苗的抗瘤效应。方法:昆明鼠60只,随机分为2组,每组30只。实验组:取6-12周龄的昆明鼠背部皮下接种PDT法产生的疫苗,每3天注射一次,每次注射50μl(相当于3×105个细胞),连续两周。一周以后再注射H22肿瘤细胞悬液0.1ml(1×106个细胞);对照组:每周每次注射50μl生理盐水,连续两周。一周以后再注射H22肿瘤细胞悬液0.1ml(1×106个细胞)。比较两组的抑瘤率和生存率。结果:实验组小鼠的抑瘤率、生存率均较对照组有显著提高。结论:PDT法产生的抗小鼠H22肝癌疫苗具有明显的抗瘤效应,该方法有望成为一种临床治疗肿瘤的新方法。

Objective： To test the contribution of the direct effects of PDT on tumor cells, we examined the immunogenicity of PDT-generated murine tumor cell lysates in a preclinical vaccine model. Methods： Sixty Kunming mice （H22 tumor host） were divided into two groups randomly and equally. Six to twelve-week-old animals were vaccinated intradermally on the right shoul der with 50μl of lysates （3 × 10^5 cell equivalents） for experimental group or medium control for control group every three days during two weeks. The animals were rested a week and then inoculated on the flank with 1 × 10^6 tumor cells harvested from ex ponentially growing cultures. And then we compared antitumor rate and survival rate between two groups. Results： Comparing with control group, antitumor rate and survival rate in experimental group increased significantly. Conclusions： Our studies suggest that the direct tumor effects of PDT play an important role in enhancing host antitumor immune response, thus PDT- generated vaccines may have well clinical potential as an adjuvant therapy.