来曲唑对大鼠子宫内膜αvβ3和HOXA10表达的影响

Effects of Letrozole on Expression of Integrin α_vβ_3 and HOXA10 in Rat Endometrium

目的:探讨应用来曲唑促排卵对大鼠围着床期子宫内膜整合素αvβ3和HOXA10表达的影响。方法:将60只Wistar雌鼠随机等分为3组:来曲唑组(letrozole,LE组),克罗米芬组(clomiphenecitrate,CC组),生理盐水组(NS组),分别进行促排卵。用免疫组化法观察大鼠围着床期子宫内膜腺上皮整合素αvβ3的表达变化;应用RT-PCR和Westenblot方法观察大鼠围着床期子宫内膜HOXA10表达情况。结果:LE组大鼠子宫内膜αvβ3表达与NS组无差异(P>0.05),但LE组显著高于CC组(P>0.01);NS组HOXA10表达高于LE组(P>0.05),LE组HOXA10表达高于CC组(P>0.01)。结论:来曲唑促排卵对大鼠子宫内膜容受性的抑制作用比克罗米芬小,有可能成为较为合适的促排卵药物之一。

Objective： To investigate the effects of letrozole on expression of integrin αvβ3 and HOXA10 on endometrium in rats. Methods： Sixty sexually mature female Wistar rats were randomly divided into 3 groups： LE group was given letrozole （2 mg/kg × 2 d）, CC group was given clomiphene citrate（100 μg/kg × 2 d）, and NS group was given 0.9% NS of the same volume. Immunohistochemical of ABC methods using monoclonal antibody against integrins αvβ3 were performed on endometriurrL Reverse transcription polymerase chain reaction （RT-PCR） method and Western blot were utilized to detect and quantitate HOXA10 mRNA expression levels in the endometrium. Results： The expression ofintegrins αvβ3 in CC group was significantly lower than those in LE group and NS group （P〈0.01）. HOXA10 expression in NS group was significantly the highest among 3 groups （P〈0.05）, and that in LE group was higher than that in CC group （P〈0.01）. Conclusion： In rats, letrozole has less suppression on endometrial receptivity than clomiphene. In the future, letrozole may act as an appropriate drug in ovulation induction.