螺旋藻氢酶的制备及其特性的研究

PREPARATION AND PROPERTIES OF HYDROGENASE FROM SPIRULINA PLATENSIS

氢是理想的不污染环境的能源,在世界能源日趋紧张的情况下,氢代谢这一研究课题受到了许多学者的重视。藻类中一些绿藻可以放氢已有不少研究;关于蓝藻的放氢,特别是不具有异形胞蓝藻的放氢研究目前报道得很少。螺旋藻是一种不能固氮的丝状蓝藻,但具有氢酶。顾天青等已进行了螺旋藻整体细胞放氢特性的研究,证明了氢酶与硝酸还原酶之间存在还原能力的竞争。在此基础上,我们提取了螺旋藻的氢酶,在分子水平上对它的放氢特性进行了研究,并以Na_2S_2O_4还原的甲基紫精作为催化放氢的试验。

Hydrogenase exists in Spirulina platensis. This paper deals with the isolation and purification of hydrogenase from Spirulina platensis. Hydrogenase from Spirulina platensis can catalyze the evolution of hydrogen from methyl viologen reduced by Na2S2O4. The optimum pH for hydrogen-evolution is about pH 8, and optimum temperature ranges from 35-45℃. Hydrogenase from Spirulina platensis is very sensitive to O2 and its cell-free preparation by differential centrifugation has different hydrogen-evolution activity in light and in dark. The activity of hydrogen-evolution increased when light was given, and the factor which can promote hydrogen-evolution was isolated.