中国西北部5个民族群体Y染色体遗传分析(英文)

Genetic analysis of Y-chromosomes in five ethnic groups from Northwest China

研究了来自中国西北部甘肃省的5个民族群体的403个男性个体的Y染色体遗传特征,其中3个为甘肃省特有少数民族:东乡族(133人)、裕固族(56人)、保安族(78人).另外,还有回族(66人)和汉族(70人).研究共涉及Y染色体上的17个遗传多态位点,其中1个是微卫星(DYS19).另外,有16个位点是单核苷酸多态位点,包括M9,92R7,SRY1532,Tat,LLY22g,SRY2627,RPS4Y,12F2,SRY8299,SY81,M20,L1Y,SRY+465,47Z,APT和DYS287(YAP).除了插入片段多态位点DYS287(YAP)使用琼脂糖直接检测外,其余位点均采用PCR-RFLP方法研究.在所研究的5个群体中,仅有7个单核苷酸M9,92R7,SRY1532,RPS4Y,12F2,LIY和DYS287(YAP)表现为多态,且构成了9种单倍群(单倍群HG1,HG2,HG3,HG4,HG9,HG10,HG13,HG16和HG26).其中:回族拥有全部9种类型,而东乡族和汉族拥有8种,保安族和裕固族占据7种类型.

Variations of seventeen Y-chromosomal DNA polymorphisms, one microsatellite （DYS19）, and sixteen biallelic markers were studied in 403 males from Northwest China. 133 individuals from Dongxiang, 56 males from Yugu and 78 individuals from Bao＇an were selected to evaluate the degree of differentiation in this region, and males from neighboring areas such as the Hui （66 males） and Han （70 males） populations were also typed. For single nucleotide polymorphism （SNP） analysis, a method using the RFLP technology was applied： In addition to these 15 SNPs, the Y-chromosomal bialIelic Alu repeat insertion DYS287 （YAP） was tested by agarose gel electrophoresis. The result shows that, among the 16 biallelic SNP/Alu systems, only seven markers, including M9, 92R7, SRY1532, RPS4Y, 12F2, L1Y and DYS287 （YAP） were found to be polymorphic in the five population samples except tat, LLY22g, M20, SRY＋465, Apt, SRY8299, SYS1, 47z, and SRY2627, for their diversities were absent in the five populations. A total number of nine different haplogroups （1, 2, 3, 4, 9, 10, 13, 16 and 26） for Y-chromosomal biallelic polymorphisms were identified among the five populations studied. Of these, nine haplogroups were present in Hui＇s, eight in Dongxiangs and Han respectively, seven in Bao＇ans and Yugus respectively.