一种分子内电荷转移荧光探针与牛血清白蛋白相互作用的研究

Study on the Interaction between ICT Fluorescence Probe and Bovine Serum Albumins

应用荧光光谱法研究了分子内电荷转移荧光探针1-酮-2-(对二甲氨基苯亚甲基)-四氢萘(KDTN)与牛血清白蛋白(BSA)的相互作用。研究表明,KDTN能与牛血清白蛋白形成稳定的络合物从而猝灭BSA的荧光,疏水作用是结合反应的主要作用力,二者的结合常数为3.2742×104L.mol-1,结合位点数n=0.9389(30℃时)。同时考察了KDTN的加入对BSA构象的影响。

The present article studied the interaction between intramolecular charge transfer fluorescence probe-l-keto-2-（p-dimethylaminobenzal）-tetrohydronaphthalene （KDTN） and bovine serum albumins （BSA）. With the concentration of KDTN increasing, the fluorescence of BSA rapidly quenched and the fluorescence peak gradually blue-shifted. The result indicated that they were bound mainly by hydrophobic interaction. The binding sites is 0. 94 （3 ℃） and the equilibrium constant K is 3.27×10^4L.mol-1, Temperature increment is advantageous to the combination. It is a single static quenching process that the fluorescence of BSA quenches, which is induced by the combination of KDTN and BSA. Further study showed that different substances had different effects on the combination of KDTN and BSA.