摘要
目的:分析肾小球足突细胞黏蛋白(Podoplanin),肿瘤内皮标记物-8(TEM-8),血管内皮生长因子D(VEGF-D)在结、直肠癌中的表达及其意义。方法:用实时定量PCR方法分析30结、肠癌标本中TEM-8,Podoplanin及VEGF-D的表达,并结合病理学分期,淋巴结转移等肿瘤特点进行分析。结果:Podoplanin在肿瘤组织中的表达较正常组织中高,并与淋巴结转移及肿瘤分期相关;VEGF-D在肿瘤组织中的表达较正常组织中高,并与淋巴结转移相关,而与肿瘤分期无明显相关性;TEM-8在正常组织中基本无阳性表达,而在结、直肠癌组织中阳性表达率为90%,与肿瘤分期,淋巴结转移相关。结论:TEM-8,Podoplanin,VEGF-D均可作为结、直肠癌的标记物,并可反映淋巴结转移状况,TEM-8更可作为肿瘤特异性标记物,在结、直肠癌的诊断、治疗、预后判断中可望发挥重要作用。
OBJECTIVE： To analyze the expression of Podoplanin, TEM-8 and VEGF-D in coloreetal cancer as well as its significance. METHODS： The expression of Podoplanin, TEM-8 and VEGF-D in coloreetal cancer specimens obtained from 30 colorectal cancer patients was analyzed using RT-PCR combining the characteristics of tumor in pathological staging and rnetastasizing of lymph nodes etc. RESULTS： Podoplanin level was higher in the tumor tissues than in normal tissues, and it was related to both lyrnphnode metastasis and tumor stage; VEGF-D level were higher in tumor tissues than in normal tissues, and it was related with lymphnode metastasis but independent of tumor stage. However, TEM-8 was negatively expressed in normal tissues, but its expression rate in colorectal cancer tissues reached 90% , and it was related to both tumor stage and lyrnphnode metastasis. CONCLUSION： Podoplanin, VEGF-D and TEM-8 can act as tumor markers for colorectal cancer and a predictor of lymph node metastasis, as well as a tumor specific marker, and which are expected to play an important role in the diagnosis, prognostic prediction and treatment of colorectal cancer.
出处
《中国医院用药评价与分析》
2008年第4期284-286,共3页
Evaluation and Analysis of Drug-use in Hospitals of China
