丛枝菌根真菌对桑扦插苗生长的影响研究

Effects of AM Fungi on the Growth of Morus Cuttings

采用温室盆栽的方法研究灭菌土中接种丛枝菌根真菌Glomus mosseae对桑扦插苗生长的影响.接种量为30个孢子菌剂(T1)、60个孢子菌剂(T2)和灭菌菌剂(CK).培育15 d统计发芽率,各处理均达到80%以上,接种丛枝菌根真菌对桑扦插苗的发芽率的影响不显著.培育30 d统计成活率,各处理分别为81.43±0.2%、84.67±0.1%、68.33±0.3%,接种丛枝菌根真菌能够显著提高扦插苗的成活率.培育90 d取根样制片观察,丛枝菌根真菌能够侵染扦插苗根系,T1,T2,CK的菌根侵染率分别为51.31±2.5%、69.23±2.2%和0,不同的接种处理,丛枝菌根真菌的侵染率不同.接种丛枝菌根真菌的处理后,主根长度、须根长度、须根数量、地下部分鲜质量和干质量都显著高于对照;T2的各项生长指标综合表现要好于T1.

Potted cultivation in greenhouse was designed to study the effects of Glornus rnosseae on the growth of Morus cuttings in sterilized soil. Inoculants of T1 , T2 and CK were mixtures made up of hyphae, spores, arbuscular mycorrhizal root segments and soil grains which contained 30, 60 and 0 spores, respectively, for the three treatments. As recorded after 15 days of culture, the germination rate of the cuttings was above 80% in all the three treatments, indicating that inoculation of AM fungi had no significant effect on the germination rate of Morus cuttings. As recorded after 30 days of culture, the survival rates of the cuttings were 81.43 ±0.2%, 84.67± 0. 1% and 68.33±0. 3%, respectively, which indicates that inoculation of AM fungi can significantly increase the survival rate of Morus cuttings. Root samples were taken and analyzed 90 days after the treatment and the results suggested that AM fungi can infect Morus roots to form arbuscular mycorrhizae. The mycorrhizal infection rate of T1 , T2 and CK was 51.31 ± 2.5%, 69.23±2.2% and 0, respec＇tively, which shows that infection rate varied with inoculation treatments. Statistical analysis of growth parameters （root length, total length of fibrous root, fibrous root number, root fresh weight and root dry weight） indicated that inoculation with AM fungi significantly improved the growth of Morus cuttings compared with that of the non-mycorrhizal control, and the effect on growth of the cuttings in T2 was better than in T1.