黄芪、全蝎、红花、丹参、葛根混合剂抑制自发性高血压大鼠阻力血管肥厚

The Effect of Naoxintong on the Resistance Vessels Hypertrophy in Spontaneously Hypertensive Rats

目的观察黄芪、全蝎、红花、丹参、葛根混合剂(脑心通)对自发性高血压大鼠(SHR)阻力血管结构和血压的影响。方法选择12周龄雄性 SHR 大鼠(二级)32只,随机分成4组,脑心通大剂量组[1.5 g/(kg·d)]、脑心通小剂量组[0.5 g/(kg·d)]、氯沙坦组[30 mg/(kg·d)]和 SHR 组(给等量蒸馏水)。另选周龄、性别相匹配的Wistar Kyoto(WKY,给等量蒸馏水)大鼠8只。各组每日灌胃给药。12周后尾袖法测定动脉血压,称质量后处死。HE 染色,计算机图像分析计算血管壁腔比,以观察各组大鼠肠系膜三级动脉的结构变化。结果 1)大、小剂量脑心通治疗后,两组 SBP 均显著低于 SHR 组[脑心通大剂量:(153.6±12.2)比脑心通小剂量:(159.2±5.8)比 SHR:(178.6±12.0)mmHg,P<0.01];大剂量脑心通组 SHR 治疗后 SBP 较治疗前明显降低[(153.6±12.2)比治疗前(163.6±11.8)mmHg,P<0.01],与小剂量组比较能更进一步降低血压(P<0.05)。2)与 SHR 组相比,小剂量脑心通治疗对肠系膜三级动脉血管的管壁面积/管腔面积及管壁厚度/管腔半径比值无影响,而大剂量脑心通可显著降低肠系膜三级动脉血管的管壁面积/管腔面积(W/L:脑心通大剂量:0.29±0.01比脑心通小剂量:0.37±0.06比SHR:0.44±0.04,P<0.01)及管壁厚度/管腔半径(Tw/RI:脑心通大剂量:0.12±0.01比脑心通小剂量:0.17±0.03比 SHR:0.20±0.02,P<0.01)。结论脑心通可剂量依赖地抑制 SHR 大鼠血压的发展,减轻 SHR 阻力血管的肥厚。

Objective To observe the effect of Naoxintong （NXT）, one of Chinese traditional herbal composition, on the resistance vessels hypertrophy in spontaneously hypertensive rats （ SHR）. Methods Thirty-two twelve-week-old male SHR were randomized to high-dose NXT treated group [NH group, 1.5 g/（ kg · d）, n= 8], low-dose NXT treated group [NL group, 0. 5 g/（kg · d）, n=8-1, losartan treated group [LOS group, 30 mg/（kg · d）, n =8-1 and distilled water control （SHR group, n=8）. Wistar-Kyoto rats（WKY group, n=8） were served as normal control. Naoxintong were administered for 12 weeks by gavage in mixture with 2 mL distilled water, SHR and WKY control were received the same amount of distilled water. Systolic blood pressure（SBP） was measured by the tail-cuff method. Wall-to-lumen area ratios of mesenteric arteries （ 3th branch） were calculated. Results 1 ） After 12 weeks treatment with high-dose and low-dose NXT, SBP in both groups was markedly lower than that in SHR control group （NH ： 153.6 ± 12. 2 vs NL： 159.2 ± 5.8 vs SHR： 178.6 ± 12.0 mmHg, P〈0.01）. High-dose NXT slightly, but statistically significantly decreased SBP（ 153.6±12. 2 vs baseline： 163.6±11.8 mmHg, P〈0.01 ）. 2 ） Wall-to-lumen area ratio（ W/L） and the thickness of the wall to the radius of the lumen（ Tw/RI） in mesenteric arteries （ 3th branch） were significantly reduced in high-dose NXT group than in SHR group（ W/L, NH：0.29±0.01 vs NL：0.37±0. 06 vs SHR：0.44＋0.04,P〈0.01; Tw/RI, NH：0.12±0.01 vs NL：0. 17±0.03 vs SHR： 0.20± 0.02, P〈0.01）. Conclusion NXT attenuates the resistance vessels hypertrophy in SHR, which may be the mechanism by which Naoxintong prevents the progression of arterial blood pressure.