养颜祛斑颗粒抗D-半乳糖所致小鼠皮肤衰老作用

Experimental Study of Anti-aging Effect of Yangyan Quban Particles on Aging Model Mice’s Skin Caused by D-galactose

目的探讨内服养颜祛斑颗粒抗皮肤衰老作用及机制。方法昆明种雌性小鼠每日背部皮下注射D-半乳糖1000mg/(kg·d)造成小鼠衰老模型,同时按高、中、低剂量(6.68、3.34、1.67g/kg)分别灌服养颜祛斑颗粒,42d后测定小鼠背部皮肤组织匀浆中谷胱甘肽过氧化物酶(GSH-Px)活性及血清中雌二醇(E2)的含量,并考察对小鼠子宫、卵巢、皮肤干重、湿重及水摄取量的影响。结果与衰老模型组比较,养颜祛斑颗粒给药组小鼠皮肤中GSH-Px活力及E2含量明显升高,尤以中剂量为最佳(P<0.05,P<0.01),而对小鼠子宫、卵巢、皮肤干重、湿重及水摄取量有增加趋势。结论养颜祛斑颗粒对D-半乳糖所致衰老小鼠皮肤有明显的抗衰老作用。

Objective To investigate the mechanism of anti-aging effect of Yangyan Quban particles on the aging skin. Methods With the dose of D-galactose 1000 mg/（kg · d） by sc administration to the female mice for 42 days, the aging model was established. The mice received Yangyan Quban particles （6.68, 3.34, 1.67 g/kg） by ig administration at the same time of injecting D-galactose. Forty-two days later, the dorsal skin samples were collected to determine glutathione peroxidase （GSH-Px） activity and serum estradiol （E2） contents, and the influence on uterus, ovary, skin dry and wet weight and the amount of water intake were studied. Results Compared with the aged model mice, the GSH-Px activity and E2 contents in the mice treated with Yangyan Quban particles were increased remarkably, especially the medium is the best dose （P〈0.05, P〈0.01）, while the uterus, ovary, skin dry and wet weight and intake of water had the increase tendency. Conclusion Yangyan Quban particles has obvious anti-aging effects on aging mice＇s skin.