助孕胶囊对多囊卵巢大鼠卵巢组织白血病抑制因子表达的影响

Effect of Zhuyun Jiaonang on the Expression of Leukemia Inhibitory Factor in Polycystic Ovary Syndrome Rats

目的观察助孕胶囊对多囊卵巢(PCO)大鼠生殖内分泌激素、卵巢白血病抑制因子(LIF)表达的影响,分析其可能的作用机制。方法采用孕激素联合人绒毛膜促性腺激素(HCG)造模法制备PCO模型大鼠。随机分为对照组、PCO模型组、PCO模型组+助孕胶囊组(中药组)、PCO模型组+克罗米酚组(西药组),采用放免法测定血性激素水平的变化,光镜观察卵巢、卵泡发育的情况,免疫组化法检测卵巢组织LIF的表达水平。结果助孕胶囊可使PCO大鼠的颗粒细胞层增厚,卵泡膜细胞层变薄,血清雄激素(T)、黄体生成素(LH)水平均明显下降,E2、FSH水平上升,并上调卵巢组织LIF的表达(P<0.05)。结论助孕胶囊能够降低PCO大鼠血清中T、LH水平,升高E2、FSH水平,上调卵巢组织LIF的表达,从而促进卵泡的发育、成熟及排卵。

Objective To observe the effect of Zhuyun Oiaonang （ZYJN） on procreate endocrine hormone and the expression of leukemia inhibitory factor （LIF） on ovaries in rat of PCO, and analyze the possible therapeutic mechanism. Methods The PCO rat models were set by pregnancy hormone combine with human chorionic gonadotrophin （HCG）, randomly divided into four group, including control group, model group, Chinese crude drug group （CCDG） and western medicine group （WMG）. The levels of serum gonadal hormone were detected by radioimmunoassay. The effects of ZYdN on ovaries and ovarial follicular development were observed by light microscope. The expressed level of LIF was detected by using the immunohistochemical staining technique. Results ZYJN could increase the thickness of granular cell layer ovary and decrease the thickness of theca cell layer. ZYdN greatly decreased the level of T and LH, increased the Level of E2 and FSH in serum, and increased the expression of LIF in ovary （P〈0.05）. Conclusion ZYdN has effect of decreasing the level of T and LH and increasing the expression of LIF in ovary adjusting genitical gland and promoting the mature and ovulation of follicular.