不同部位及生长年限云南红豆杉中紫杉醇含量的测定

Content Determination of Taxol in Taxus Yunnanensis of Different Parts and Growth Time Limits

目的研究云南红豆杉不同部位中紫杉醇的含量以及不同生长年限对云南红豆杉枝叶中紫杉醇含量的影响。方法采用HPLC法,HypersilODS2色谱柱,流动相为甲醇-乙腈-水(20∶30∶50),柱温30℃,检测波长227nm,比较云南红豆杉的树皮、针叶、小枝和树根中紫杉醇的含量,以及不同生长年限对云南红豆杉枝叶中紫杉醇含量的影响。结果云南红豆杉树皮中紫杉醇的含量最高,根中最少,小枝、针叶中含量较高;随着生长年限的增加,云南红豆杉枝叶中紫杉醇的含量逐渐增加。结论云南红豆杉枝叶的采摘以5年生以上为佳。

Objective To study the content of taxol in different parts of Taxus Yunnanensis and the effects of different growth time on taxol content. Method A HPLC method was developed for the detetmination of taxol content in bark, needle, twig, root from Taxus Yunnanensi, and the effects of different growth time. Results Taxol content in bark was the highest, that in root was the lowest and that in needle and twig were higher. Taxol content was higher with the growth time in needle and twig of Taxus Yunnanensi. Conclution It was significant to take taxol from needle and twig of Taxus Yunnanensis which is more than five years growth time.