RP—HPLC法测定大剂量化疗时人血浆中甲氨喋呤浓度

Determination of methotrexate in high dose chemical therapy in human plasma by RP-HPLC

目的 建立大剂量化疗时人血浆中甲氨喋呤浓度的测定方法。方法 采用反相高效液相色谱法（RP—HPLC）。色谱柱：μ Bondapak C18（4．6×150mm），柱温：35℃，流动相为甲醇：（pH7．2）磷酸缓冲液（17：83，v／v），流速：1．0ml·min^-1，检测波长306nm。结果 线性范围0．08～100μg·ml^-1，线性关系良好，r=0．9999，最低检测浓度0．01μg·ml^-1，回收率102．0％，日内及日间RSD均〈5％。结论 该方法简单快捷、灵敏度高、结果准确，适用于大剂量化疗时甲氨喋呤血药浓度监测。

Objective To establish the method to determine of methotrexate in chemical therapy in hunan plasma. Methods The concentration of MTX the sample was determined by RP HPLC. μ Bondapak C18（4.6×150mm） was used. The mobile phase consisted of methanol：（ pH7. 2）phosphate solution （17：83, V/V）.at a flow rate of 1.0 ml·min^-1.The column temperature was 35 ℃ and wavelength was set at 306nm. Results Good linearity was shown over the concentration range of 0.08-100 μ g·ml^-1（r=0.9999）.The minimal detectable methotrexate drug concentration was 0.01μg·ml^-1.The average recovery was 102.0%.The relative standard deviatin of within day and between day were less than 5%.Conclusion This method is concise, convenient, high sensitive and accurate to monitor the concentration of MTX in plasma.