应用末端标记限制性片段长度多态性技术分析胆固醇结石内的菌群构成

The Application of Terminal Restriction Fragment Length Polymorphism for Analysis of Different Components of the Gall-stone Bacterium Community

末端标记限制性片段长度多态性是一种新兴的微生物生态学技术,以胆囊内的微生物群落16S rDNA为目标,利用此技术对胆囊结石患者体内结石和胆汁的微生物群落结构进行定性和定量分析,根据分析结果与正常人体内的胆汁进行比较,从而阐明胆结石患者与正常人体胆囊内微生物群落结构的差异性,为进一步预防和控制胆囊结石的发生提供有用的依据。

Terminal restriction fragment length polymorphism is a new microbioecology technique. It is used to make a qualitative and quantitative analysis of the different components in the gall-stone and bile bacterium community,with a focus on the 16S rDNA of microorganism in gallbladder. By comparing the results between cholecystolithiasis patient and normal person, the difference is drawn, which will offer available basis for preventing and controlling the occurrence of cholecystolithiasis.