摘要
目的探讨hvWF A3-Flag-GPI融合蛋白设计的合理性,以期获得能够锚定至内皮祖细胞表面,引导细胞黏附于受损血管壁的分子。方法应用Gene Construction Kit2.5,www.expasy.com网站提供的分析方案,分析重组体的开放读框及融合蛋白的柔性,并作了蛋白质二级结构模拟。结果重组体Flag linker所在部位柔性高,融合蛋白表达后,二级结构预测Flag lin-ker不改变蛋白结构,完全符合作者设计hvWF A3-Flag-GPI融合蛋白的初衷。结论重组蛋白设计合理,融合蛋白有很大可能保留了hvWF A3和GPI的理化特性,为获得引导EPCs特异归巢至受损血管壁的相关分子提供前期基础。
Objective To study the reasonability of designation of hvWF A3-Flag-GPI fusion protein. Methods Using sequence analysis software and protocols prescribed on website www. expasy, corn to analyze the open reading frame of the recombinant and the flexibility.as well as the secondary structure of hvWF A3-Flag-GPI fusion protein. Results The fusion protein had correct domains of hvWF A3-Flag-GPI. Flag linker had high flexibility and did not influence the secondary structure of fusion protein. Conclusion Computer analysis can help to rationally design the recombinant protein. The attenuated hvWF A3 and GPI protein keep their maximum biological activities.
出处
《重庆医学》
CAS
CSCD
2008年第9期924-925,共2页
Chongqing medicine
基金
国家自然科学基金资助项目(30470729)。
